Isolation and identification of microorganisms ppt to pdf
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- Isolation (microbiology)
- Crash Course in Microbial Identification
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Not a MyNAP member yet? Register for a free account to start saving and receiving special member only perks. The transmission of infectious diseases via contaminated water continues to be a risk to public health in the United States and throughout the rest of the. Source and finished drinking waters are vulnerable to microbial pathogen contamination from a variety of sources of human and animal fecal wastes and from the introduction and proliferation of nonfecal pathogenic microbes. Throughout most of the modem history of drinking water supply, concerns about pathogenic microbes have focused on enteric bacteria of human fecal origin. These concerns led to the development of criteria and standards for bacteriological quality intended to protect against excessive risks from enteric bacterial pathogens such as Salmonella typhi and other nontyphoid Salmonella spp.
Need a crash course in methods for identifying microbes? So, how do we go about identifying microbes? Traditional methods rely on phenotypic identification using staining, culturing, and simple biochemical tests. Nowadays, more powerful molecular, immunological, and biochemical analytical methods complement and sometimes replace traditional methods. Macroscopic features encompass the overall appearance of a microorganism, including its shape, size, color, and smell i.
Copy embed code:. Automatically changes to Flash or non-Flash embed. WordPress Embed Customize Embed. URL: Copy. Presentation Description No description available. Demonstrate their properties. Typing bacterial isolates.
Those that are difficult or time-consuming to isolate Rapid or high-throughput identification of microorganisms; Detection and analysis of resistance genes.
Crash Course in Microbial Identification
In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes , as present in the environment, for example in water or soil flora , or from living beings with skin flora , oral flora or gut flora , in order to identify the microbe s of interest. Historically, the laboratory techniques of isolation first developed in the field of bacteriology and parasitology during the 19th century , before those in virology during the 20th century. Methods of microbial isolation have drastically changed over the past 50 years, from a labor perspective with increasing mechanization, and in regard to the technology involved, and hence speed and accuracy.
The Annals of Work Exposures and Health recently published two interesting studies combining the use of culture and molecular methods. The method involves the cultivation of bioaerosol samples on agar media and the pick-up of grown colonies 16S rRNA gene amplification, subsequent cloning, sequencing, and identification of bacterial isolates through the assignment against known gene databases. The aim of the present paper is to discuss the contribution of the proposed method in regards with the already proposed approaches used for identification of cultured bacteria. It details the new proposed method and discusses its contribution to the existing culture-based identification methods.